Coding
Part:BBa_K4467002:Design
Designed by: Eloise Paine Group: iGEM22_UNSW_Australia (2022-10-10)
Pst_12806
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 333
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimisation was performed for expression of this part in NEB TURBO E.coli and NEB T7 Express E. coli.
Source
Pst_12806 encodes for a haustorium-specific protein from the wheat stripe rust fungus Puccinia striiformis f.sp.tritici (Pst). The genomic sequence was sourced from Xu, Q., et al. (2019).
References
XU, Q., et al. (2019). "An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function." Nature Communications 10(1): 5571.